HIsTological and CLinical effects of Imipramine in the treatment of patients with cancer over-expressing Fascin1. (HITCLIF)

2023-504943-15-00 Protocol IMIB-HTF-2021-01 Therapeutic exploratory (Phase II) Ongoing, recruiting

Start 19 Aug 2022 · Status Ongoing, recruiting · 1 EU/EEA countries · 3 sites · Protocol IMIB-HTF-2021-01

Overview

Sponsor-declared trial summary

Phase Therapeutic exploratory (Phase II)
Status Ongoing, recruiting
Participants planned 180
Countries 1
Sites 3

Cancer(colon cancer stage II-III, rectal cancer, and breast cancer)over-expressing Fascin1

To assess whether treatment with Tofranil© produces significant changes in the histological and molecular manifestations of the epithelial-mesenchymal transition (EMT) in the period from tumor diagnosis by biopsy to surgical resection. The evaluation of the Main Objective will be carried out in the histological examin…

Key facts

Sponsor
Fundacion Para La Formacion E Investigacion Sanitaria De La Region De Murcia
Participant type
Patients
Age range
18-64 years, 65+ years
Gender
Male and Female
Therapeutic area
Diseases [C] - Hormonal diseases [C19], Diseases [C] - Digestive System Diseases [C06]
Trial duration
19 Aug 2022 → ongoing
Decision date (initial)
2023-08-07
Transition trial
Yes
Low-intervention
No
Rare-disease indication
No
Vulnerable population
No

External identifiers

EU CT number
2023-504943-15-00
EudraCT number
2021-001328-17

Trial design

CTIS Part I — objectives, methods, condition coding

Main objective

Scope: Efficacy, Safety

To assess whether treatment with Tofranil© produces significant changes in the histological and molecular manifestations of the epithelial-mesenchymal transition (EMT) in the period from tumor diagnosis by biopsy to surgical resection.

The evaluation of the Main Objective will be carried out in the histological examination of the tumor piece product of the surgical resection; usually at 2-6 weeks for group A and between 3-6 months for neoadjuvant patients (group B).

Secondary objectives 1

  1. To evaluate if the treatment with Tofranil© produces a decrease in the quantification of circulating mutated tumor DNA or in the serum levels of Fascina1. This secondary objective will be evaluated only if the primary objective demonstrates a beneficial effect of Tofranil©.

Conditions and MedDRA coding

Cancer(colon cancer stage II-III, rectal cancer, and breast cancer)over-expressing Fascin1

VersionLevelCodeTermSystem organ class
20.0 PT 10038038 Rectal cancer 100000004864
21.0 PT 10009955 Colon cancer stage III 100000004864
20.0 PT 10006187 Breast cancer 100000004864
21.0 PT 10009954 Colon cancer stage II 100000004864

Eligibility criteria

Principal inclusion / exclusion criteria as submitted by sponsor

Inclusion criteria 5

  1. Resectable tumor.
  2. Obtaining Written Informed Consent.
  3. Diagnosis of colon, rectal or triple negative breast cancer by biopsy.
  4. Overexpression of fascina1 in primary tumor.
  5. Patient over 18 years of age

Exclusion criteria 10

  1. Metastatic disease at diagnosis or not candidates for neoadjuvant therapy.
  2. Colorectal cancer with clinical symptoms of sub-obstruction or obstruction.
  3. Known diagnosis of major depressive disorder, bipolar depression, or psychosis.
  4. ECOG scale 3 or 4.
  5. Severe renal failure (GFR <30 mL/min).
  6. Hepatic failure or bilirubin > 2 mg/dl.
  7. Suspected inability to comply with treatment adequately or conditions that limit its reliability (psychiatric illness, drug addiction, etc.).
  8. History of heart disease.
  9. Patient on current treatment with SSRIs, SNRIs, MAOIs, tramadol or trazadone or within the 14 days prior to recruitment.
  10. Woman of childbearing age who refuses to use any contraceptive method.

Endpoints

Primary and secondary outcome measures (English text)

Primary endpoints 1

  1. Changes in the invading front associated with the epithelial-mesenchymal transition quantified as measurement of tumor budding, cytoplasmic pseudofragments, pattern of tumor invasion, and characteristics of the immune infiltrate (intra- and peritumoral lymphocytes, characterization of Crohn-like response macrophages).

Secondary endpoints 1

  1. Evaluation of plasma quantification of tumor DNA copies and the expression of serum and tissue fascina1 in the surgical resection piece.

Investigational products

Investigational medicinal products (IMPs), comparators, placebo, auxiliary

Test 1

TOFRANIL 50 mg comprimidos recubiertos

PRD791264 · Product

Active substance
Imipramine Hydrochloride
Pharmaceutical form
COATED TABLET
Route of administration
ORAL
Max daily dose
195 mg milligram(s)
Max total dose
30745 mg milligram(s)
Max treatment duration
24 Week(s)
Authorisation status
Authorised
ATC code
N06AA02 — IMIPRAMINE
Marketing authorisation
48.384
MA holder
AMDIPHARM LIMITED
MA country
Spain
Paediatric formulation
No
Orphan designation
No
Modified vs. Marketing Authorisation
Yes
Modification description
The Pharmacy Service of the Hospital General Universitario Santa Lucía will be responsible for manufacturing the medication for the trial. The preparation of the trial medication will be carried out by qualified personnel in the pharmacotechnics white room of the Pharmacy Service following the SOP of Annex I, II, III and IV. The goal of this process is to ensure that the dosage form of the investigational drug (IM) is the same as the placebo and that the two are indistinguishable. The MI consists of imipramine capsules of 2 different doses: 25 mg and 65 mg. For the elaboration of the MI, Tofranil® 50mg tablets will be used, which will be disintegrated and encapsulated at a rate of 25 or 65 mg per capsule in each case. The capsules will be manufactured in batches of 200 capsules. In each batch, a mass uniformity quality control will be carried out by weighing and a batch number will be assigned. Regarding the expiration date given to the capsules, an evaluation of the impact that manufacturing and reconditioning could have on the stability of the final preparation has been carried out using the Risk Matrix for Non-Sterile Preparations of the Correct Manufacturing Standards Guide. Medicines for Human and Veterinary Use (Annex V). For the elaboration of the placebo, the same excipients will be encapsulated as in the MI and in the same proportions. Both MI and placebo will be encapsulated in No. 3 hard gelatin capsules. and no. 4 opaque red. The final weight and appearance in both preparations will be the same.

Placebo 1

Sucrose c.s. and lactose c.s.

N/A · Product

Other product name
N/A
Pharmaceutical form
N/A
ATC code
N/A — N/A
Marketing authorisation
N/A
MA holder
N/A
MA country
Iceland
Paediatric formulation
No

Sponsors and contacts

Sponsor organisations, regulatory contacts, third parties

Fundacion Para La Formacion E Investigacion Sanitaria De La Region De Murcia

Sponsor organisation
Fundacion Para La Formacion E Investigacion Sanitaria De La Region De Murcia
Address
Carretera De Cartagena S/n, El Palmar El Palmar
City
Murcia
Postcode
30120
Country
Spain

Scientific contact point

Organisation
Fundacion Para La Formacion E Investigacion Sanitaria De La Region De Murcia
Contact name
Clinical trial information desk

Public contact point

Organisation
Fundacion Para La Formacion E Investigacion Sanitaria De La Region De Murcia
Contact name
Clinical trial information desk

Locations

1 EU/EEA country · 3 investigational sites

By country

CountryMS statusPlanned subjectsSites
Spain Ongoing, recruiting 180 3
Rest of world 0

Investigational sites

Spain

3 sites · Ongoing, recruiting
University Clinical Hospital Virgen De La Arrixaca
Medical oncology service, Carretera De Cartagena Sn, El Palmar, Murcia
Hospital General Universitario Morales Meseguer
Medical oncology service., Avenida Del Marques De Los Velez S/n, 30008, Murcia
General University Hospital Santa Lucia
Pathological anatomy service., Calle Mezquita S/n Paraje Los Arcos, Santa Lucia, Cartagena

Country notifications

Trial-start, recruitment-start, end and early-termination notifications submitted per Member State

Country Trial startTrial end Recruitment startRecruitment end Early termination
Spain 2022-08-19 2022-08-19

Results and documents

Annex IV summary of results, Annex V layperson summary, and all documents registered in CTIS for this trial

Documents 7 files

Public protocol annexes, IB summaries, regulatory submissions and post-authorisation documents registered in CTIS.

TypeTitleVersion
Recruitment arrangements (for publication) Procedimiento de seleccion de pacientes 1
Subject information and informed consent form (for publication) HIPCI HITCLIF HCUVA V1 21052022 HCUVA english 1
Subject information and informed consent form (for publication) HIPCI HITCLIF IHQ V2 22062022 2.0
Subject information and informed consent form (for publication) HIPCI HITCLIF IHQ V2 22062022 english 2.0
Subject information and informed consent form (for publication) HIPCI HITCLIF V1 del 21052021 1
Subject information and informed consent form (for publication) V5 HIPCI Biobanco Murcia 5
Subject information and informed consent form (for publication) V6 HIPCI Biobanco Murcia 6

Application history

3 submissions — initial application plus substantial / non-substantial modifications

#TypeCodeSubmittedReference MSConclusionDecision date
1 INITIAL IN 2023-07-31 Spain Acceptable
2023-08-07
2023-08-07
2 SUBSTANTIAL MODIFICATION SM-1 2023-11-02 Spain Acceptable
2024-01-15
2024-01-15
3 SUBSTANTIAL MODIFICATION SM-2 2025-05-22 Spain Acceptable 2025-06-13