Quercus ilex and Quercus robur allergen extracts. Determination of in the vivo histamine equivalent prick test units (HEP).

2024-515189-14-00 Protocol T525-STD-043 Therapeutic exploratory (Phase II) Ended

Start 26 Jan 2023 · End 28 Jan 2025 · Status Ended · 1 EU/EEA countries · 1 sites · Protocol T525-STD-043

Overview

Sponsor-declared trial summary

Phase Therapeutic exploratory (Phase II)
Status Ended
Participants planned 30
Countries 1
Sites 1

Allergy to Quercus ilex and Quercus robur

The main objective is to evaluate the concentration of allergenic extract from Quercus ilex and Quercus robur that causes a papule of a size equivalent to that produced by a solution of histamine dihydrochlorohydrochloride at 10 mg/mL.

Key facts

Sponsor
Inmunotek S.L.
Participant type
Patients
Age range
18-64 years
Gender
Male and Female
Therapeutic area
Diseases [C] - Immune System Diseases [C20]
Trial duration
26 Jan 2023 → 28 Jan 2025
Decision date (initial)
2024-10-03
Transition trial
Yes
Low-intervention
No
Rare-disease indication
No
Vulnerable population
No
Funding sources
INMUNOTEK, S.L.

External identifiers

EU CT number
2024-515189-14-00
EudraCT number
2020-005388-30

Trial design

CTIS Part I — objectives, methods, condition coding

Main objective

Scope: Dose response, Diagnosis

The main objective is to evaluate the concentration of allergenic extract from Quercus ilex and Quercus robur that causes a papule of a size equivalent to that produced by a solution of histamine dihydrochlorohydrochloride at 10 mg/mL.

Conditions and MedDRA coding

Allergy to Quercus ilex and Quercus robur

VersionLevelCodeTermSystem organ class
20.1 PT 10053462 Allergy test 100000004848

Eligibility criteria

Principal inclusion / exclusion criteria as submitted by sponsor

Inclusion criteria 7

  1. Subjects with a positive clinical history of inhalant allergy (rhinitis and/or rhinoconjunctivitis and/or asthma) to Quercus ilex and Quercus robur.
  2. Subjects with a positive n prick-test (mean papule diameter >= 3 mm) with an extract of the same allergen and/or presence of specific IgE to that allergen.
  3. The mean papule area obtained with Histamine dichlorohydrate at 10 mg/mL should be >= 7 mm2.
  4. Age: between 18 and 64 years.
  5. Both sexes.
  6. Must be able to give informed consent.
  7. Women of childbearing age (from menarche) must present a negative urine pregnancy test at the time of entry into the trial.

Exclusion criteria 12

  1. Subjects outside the age range.
  2. Subjects who have previously received immunotherapy within the last 5 years for the treatment of allergic asthma or allergen-induced allergic rhinoconjunctivitis that may interfere with Quercus ilex and Quercus robur extract.
  3. Subjects with significant symptoms of rhinoconjunctivitis and/or bronchial asthma in whom discontinuation of systemic antihistamine treatment is contraindicated.
  4. Subjects who have previously had a severe secondary reaction during diagnostic skin prick tests.
  5. Subjects under treatment with ß-blockers.
  6. Subjects who are clinically unstable (acute asthma, febrile, etc.).
  7. Subjects with active urticaria lesions, severe active dermographism, severe atopic dermatitis, sunburn, eczema, psoriasis lesions in the area where the prick test is performed (risk of false positives).
  8. Subjects with active herpes simplex virus infection or varicella zoster herpes in the area where the prick test is performed.
  9. Subjects with any pathology in which the administration of adrenaline is contraindicated (hyperthyroidism, hypertension, heart disease, etc.).
  10. Conditions in which the subject is unable to cooperate and severe psychiatric disorders.
  11. Pregnant women or women at risk of pregnancy and breastfeeding women.
  12. Subjects with known allergy to phenol.

Endpoints

Primary and secondary outcome measures (English text)

Primary endpoints 1

  1. The primary and single variable is the size of the induced skin papule on application of each of the concentrations of the allergenic extracts and of the positive (histamine) and negative controls, using the prick test.

Investigational products

Investigational medicinal products (IMPs), comparators, placebo, auxiliary

Test 2

Prick test of Quercus robur allergen extract

PRD11535540 · Product

Active substance
Quercus Robur Pollen Extract
Pharmaceutical form
SOLUTION FOR SKIN-PRICK TEST
Route of administration
CUTANEOUS USE
Max daily dose
2 Gtt drop(s)
Max total dose
6 nl nanolitre(s)
Max treatment duration
1 Day(s)
Authorisation status
Not Authorised
ATC code
V01AA05 — TREE POLLEN
MA holder
INMUNOTEK S.L.
Paediatric formulation
No
Orphan designation
No

Prick test of Quercus ilex allergen extract

PRD11535505 · Product

Active substance
Quercus Ilex Allergen Extract
Substance synonyms
T525
Pharmaceutical form
SOLUTION FOR SKIN-PRICK TEST
Route of administration
CUTANEOUS USE
Max daily dose
2 Gtt drop(s)
Max total dose
6 nl nanolitre(s)
Max treatment duration
1 Day(s)
Authorisation status
Not Authorised
ATC code
V01AA05 — TREE POLLEN
MA holder
INMUNOTEK S.L.
Paediatric formulation
No
Orphan designation
No

Auxiliary 2

Skin Prick Test Negative control

PRD11276451 · Product

Active substance
Sodium Chloride
Pharmaceutical form
SOLUTION FOR SKIN-PRICK TEST
Route of administration
CUTANEOUS USE
Max daily dose
2 Gtt drop(s)
Max total dose
2 nl nanolitre(s)
Max treatment duration
1 Day(s)
Authorisation status
Not Authorised
ATC code
V04CL — TESTS FOR ALLERGIC DISEASES
MA holder
INMUNOTEK S.L.
Paediatric formulation
No
Orphan designation
No

Skin Prick Test Positive control

PRD11269985 · Product

Active substance
Histamine Hydrochloride
Pharmaceutical form
SOLUTION FOR SKIN-PRICK TEST
Route of administration
CUTANEOUS USE
Max daily dose
2 Gtt drop(s)
Max total dose
2 nl nanolitre(s)
Max treatment duration
1 Day(s)
Authorisation status
Not Authorised
ATC code
L03AX14 — -
MA holder
INMUNOTEK S.L.
Paediatric formulation
No
Orphan designation
No

Sponsors and contacts

Sponsor organisations, regulatory contacts, third parties

Inmunotek S.L.

Sponsor organisation
Inmunotek S.L.
Address
Calle Punto Mobi 5
City
Alcala De Henares
Postcode
28805
Country
Spain

Scientific contact point

Organisation
Inmunotek S.L.
Contact name
Miguel Casanovas

Public contact point

Organisation
Inmunotek S.L.
Contact name
Raquel Caballero

Locations

1 EU/EEA country · 1 investigational sites

By country

CountryMS statusPlanned subjectsSites
Spain Ended 30 1
Rest of world 0

Investigational sites

Spain

1 site · Ended
Clínica subiza S.A.
Allergy, Calle del general Pardiñas 116, 28006, Madrid

Country notifications

Trial-start, recruitment-start, end and early-termination notifications submitted per Member State

Country Trial startTrial end Recruitment startRecruitment end Early termination
Spain 2023-01-26 2025-01-28 2023-02-08 2024-11-26

Results and documents

Annex IV summary of results, Annex V layperson summary, and all documents registered in CTIS for this trial

Summary of results Art. 37(4) CTR

TitleSubmission dateStatusType
Quercus ilex and Quercus robur allergen extracts. Determination of the in vivo allergenic potency in
SUM-107033
2025-12-01T12:49:29 Submitted Summary of Results

Layperson summary Annex V

TitleSubmission dateStatusType
Extracto alergénico de Quercus ilex y Quercus robur. Determinación de la potencia alergénica in vivo 2025-12-01T12:49:48 Submitted Laypersons Summary of Results

Documents 7 files

Public protocol annexes, IB summaries, regulatory submissions and post-authorisation documents registered in CTIS.

TypeTitleVersion
Laypersons summary of results (for publication) Clinical Study Report_Layperson summary_SP_20250912 NA
Protocol (for publication) D1_Protocol ES_2024-515189-14-00_Redacted 03.1
Recruitment arrangements (for publication) K1_Recruitment arrangements 1
Subject information and informed consent form (for publication) L1_SIS and ICF general redacted 02
Summary of results (for publication) Clinical Study Report_Summary of results_20251106 1
Synopsis of the protocol (for publication) D1_Protocol synopsis ES_2024-515189-14-00_Redacted 03.1
Synopsis of the protocol (for publication) D1_Protocol synopsis_EN_2024-515189-14-00_Redacted 03.1

Application history

1 submissions — initial application plus substantial / non-substantial modifications

#TypeCodeSubmittedReference MSConclusionDecision date
1 INITIAL IN 2024-09-06 Spain Acceptable
2024-10-03
2024-10-03