Infusion of ex vivo-generated allogeneic natural killer cells in combination with subcutaneous IL-2 in patients with acute myeloid leukemia: a phase I/IIa study

2024-515357-16-00 Protocol HEMAML42-NK4AML Therapeutic exploratory (Phase II) Ongoing, recruitment ended

Start 20 Jan 2025 · Status Ongoing, recruitment ended · 1 EU/EEA countries · 1 sites · Protocol HEMAML42-NK4AML

Overview

Sponsor-declared trial summary

Phase Therapeutic exploratory (Phase II)
Status Ongoing, recruitment ended
Participants planned 16
Countries 1
Sites 1

MDS with excess blasts, MDS/AML or AML patients

Of phase I of the study is to evaluate the safety and toxicity of the infusion of ex vivo-expanded RNK001 NK cells, both with and without SC IL-2, following a non-myeloablative immunosuppressive conditioning regimen in patients with MDS with excess blasts, MDS/AML or AML. Of phase IIa of the study is to evaluate the e…

Key facts

Sponsor
Stichting Radboud universitair medisch centrum
Participant type
Patients
Age range
18-64 years, 65+ years
Gender
Male and Female
Therapeutic area
Diseases [C] - Neoplasms [C04]
Trial duration
20 Jan 2025 → ongoing
Decision date (initial)
2025-01-20
Transition trial
Yes
Low-intervention
No
Rare-disease indication
Yes
Vulnerable population
No
Funding sources
KWF Kankerbestrijding

External identifiers

EU CT number
2024-515357-16-00
EudraCT number
2019-001929-27

Trial design

CTIS Part I — objectives, methods, condition coding

Main objective

Scope: Therapy, Others, Efficacy, Pharmacokinetic, Safety

Of phase I of the study is to evaluate the safety and toxicity of the infusion of ex vivo-expanded RNK001 NK cells, both with and without SC IL-2, following a non-myeloablative immunosuppressive conditioning regimen in patients with MDS with excess blasts, MDS/AML or AML.
Of phase IIa of the study is to evaluate the effect of RNK001 NK cell adoptive immunotherapy in combination with SC IL-2 following a non-myeloablative immunosuppressive conditioning regime on disease activity in patients with MDS with excess blasts, AML/MDS or AML.

Conditions and MedDRA coding

MDS with excess blasts, MDS/AML or AML patients

Eligibility criteria

Principal inclusion / exclusion criteria as submitted by sponsor

Inclusion criteria 1

  1. -MDS with excess blasts, MDS/AML or AML or defined according to ELN 2022 (including MDS, MDS/AML or AML with mutated TP53); AML may be secondary to prior hematological disorders, including MDS, and/or therapy-related. - Stable or at least non-rapidly progressive disease with or without disease controlling medication. - Patients may belong to any of the following categories: o Relapsed/refractory disease after treatment with intensive chemotherapy, hypomethylating agents, targeted agents, autologous or allo-SCT (at least 6 months ago) and DLI o Newly diagnosed, untreated patients ineligible for allo-SCT - Age ≥ 18 years - WHO performance 0-2 (Appendix 2) - Life expectancy of > 4 months - Written informed consent - Hydrea is allowed as pre-treatment to control blast count until day -3 - Hypomethylating agents decitabine or azacitidine are allowed until day -7 (i.e. last administration >28 days before start chemotherapy).

Exclusion criteria 1

  1. - Rapid-progressive disease in case of previous therapy (see Appendix 1). - Patients on immunosuppressive drugs or active GvHD - Patients with active infections (viral, bacterial or fungal); acute anti-infectious therapy must have been completed within 7 days prior to study treatment - Severe cardiovascular disease (CTCAE III-IV) - Severe pulmonary dysfunction (CTCAE III-IV) - Severe renal dysfunction (CTCAE III-IV) - Severe hepatic dysfunction (CTCAE III-IV) - Severe neurological or psychiatric dysfunction (CTCAE III-IV) - Patients on concurrent chemotherapy or interferon-alpha treatment - Pregnancy or breastfeeding

Endpoints

Primary and secondary outcome measures (English text)

Primary endpoints 1

  1. During the phase I safety study, patients will be evaluated intensively for toxicity caused by the RNK001 NK cell infusions, whether or not followed by SC IL-2, using the CTCAE toxicity criteria and graft versus host disease (GvHD) classification criteria, defining dose limiting toxicities (DLTs). For phase IIa of the study, clinical response to therapy is the main study parameter and will be defined according to European Leukemia Network (ELN) response criteria by day +28 post NK cell adminis.

Investigational products

Investigational medicinal products (IMPs), comparators, placebo, auxiliary

Test 2

Proleukin 18 x 106 IE Poeder voor oplossing voor injectie of infusie

PRD11294442 · Product

Active substance
Aldesleukin
Pharmaceutical form
SOLUTION FOR INJECTION/INFUSION
Route of administration
SUBCUTANEOUS
Max daily dose
6000000 U unit(s)
Max total dose
36000000 U unit(s)
Max treatment duration
6 Day(s)
Authorisation status
Authorised
ATC code
L03AC01 — ALDESLEUKIN
Marketing authorisation
RVG 13354
MA holder
IOVANCE BIOTHERAPEUTICS B.V
MA country
Netherlands
Paediatric formulation
No
Orphan designation
No
Modified vs. Marketing Authorisation
No

RNK001

PRD11861601 · Product

Active substance
RNK001
Pharmaceutical form
DISPERSION FOR INFUSION
Route of administration
INTRAVENIOUS INFUSION
Max daily dose
3000000000 U unit(s)
Max total dose
3000000000 U unit(s)
Max treatment duration
1 Day(s)
Authorisation status
Not Authorised
MA holder
STICHTING RADBOUD UNIVERSITY MEDICAL CENTER
Paediatric formulation
No
Orphan designation
No

Sponsors and contacts

Sponsor organisations, regulatory contacts, third parties

Stichting Radboud universitair medisch centrum

Sponsor organisation
Stichting Radboud universitair medisch centrum
Address
Geert Grooteplein Zuid 10
City
Nijmegen
Postcode
6525 GA
Country
Netherlands

Scientific contact point

Organisation
Stichting Radboud universitair medisch centrum
Contact name
Trialbureau Hematologie

Public contact point

Organisation
Stichting Radboud universitair medisch centrum
Contact name
Trialbureau Hematologie

Locations

1 EU/EEA country · 1 investigational sites

By country

CountryMS statusPlanned subjectsSites
Netherlands Ongoing, recruitment ended 16 1
Rest of world 0

Investigational sites

Netherlands

1 site · Ongoing, recruitment ended
Radboud universitair medisch centrum Stichting
Hematologie, Geert Grooteplein Zuid 10, 6525 GA, Nijmegen

Country notifications

Trial-start, recruitment-start, end and early-termination notifications submitted per Member State

Country Trial startTrial end Recruitment startRecruitment end Early termination
Netherlands 2025-01-20 2025-01-20 2025-08-31

Results and documents

Annex IV summary of results, Annex V layperson summary, and all documents registered in CTIS for this trial

Documents 4 files

Public protocol annexes, IB summaries, regulatory submissions and post-authorisation documents registered in CTIS.

TypeTitleVersion
Protocol (for publication) D1_Protocol NK4AML v5 2024-515357-16-00 5.0
Recruitment arrangements (for publication) K1_blank_document_Recruitment_arrangements 0
Subject information and informed consent form (for publication) L1_SIS and ICF NK4AML 4.0
Summary of Product Characteristics (SmPC) (for publication) E2_SmPC Proleukin 0

Application history

1 submissions — initial application plus substantial / non-substantial modifications

#TypeCodeSubmittedReference MSConclusionDecision date
1 INITIAL IN 2025-01-06 Netherlands Acceptable
2025-01-20
2025-01-20